SUBmerged™ ATF P-SUB size 2 liter VV keep HFF free from fouling

Benefits of this particular P-SUBmerged are:

  • avoid HFF fouling – freedom to select broth velocities for gentle harvest and sequential pulses flushing out debris from inside the HFF
  • eliminate dead volume – skip all the hoses, traditional plastic connectors and SUBmerge the HFF
  • avoid cell death - inverted Thalia pump arranged on top of the HFF = hereby the diaphragm pump cannot accumulate cells 
  • reduce shear force – protect cells by eliminating hoses, fittings - SUBmerge the HFF inside the P-SUB and the inverted Thalia on top of HFF 
  • increased Kla values - aeration via membrane for gas diffusion - 30% better via large surface area tubular pico-sparger

Industry leading architecture P-SUB pre-assembled and ready to install:

  • 0.5 – 1.5 liter Working Volume (WV) from 2 liter Vessel Volume (VV) OD 110 mm

As this P-SUB feature an extra PG13 port these 2 SepraPor® / Thalia PG13 kit options are possible:

  • SepraPor HFF OD 9.5 x 300 mm, 110 cm2, LASER guided Thalia TM60A
  • SepraPor HFF OD 12 x 300 mm, 170 cm2, LASER guided Thalia TM60A
  • SUBmerged SepraPor unified with inverted Thalia direct connection (no Luer-Lok or TRI-clamps) for reduced shear stress
  • Inverted ground-breaking LASER guided Thalia TM60A diaphragm Single-Use-Pump (A-SUP)
  • Single-Use-Sensors (SUS) VisiWell for DO, OneFerm pH, (optional InCyte bio-mass on SUB wall with HSF), temp well, level as needed
CellRetention 2 ATF extended KLa
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CellRetention-2 ATF extended KLa

CellRetention 2 ATF tight headplate
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CellRetention-2 ATF tight headplate

CellRetention 2 ATF on HSF in MST
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CellRetention-2 ATF on HSF in MST

All three photos show the 2 liter P-SUB with OD 4 mm thin wall silicone hose Membrane-Diffusion-Cage for better KLa values compared to micro sparger. Right photo show the P-SUB mounted in the Heating-Support-Foot (HSF) bolted on the Magnetic-Stirrer-Table (MST) separating the black servo motor from the P-SUB headplate. The HSF is a perfect thermal control with sufficient contact to the P-SUB surface for accurate temperature regulation. Red LASER sensor mounts in a Re-Usable-Bracket above the dome of the inverted Thalia TM60A A-SUP.

General features of CellRetention™ 2 liter P-SUB for Head-Plate-Drive (HPD):

  • Head plate with 4 x PG13.5 circumference ports and 6 ports for In&Out with small diameter hoses, exhaust, temp well
  • Center PG13.5 occupied by HPD bearing assembly
  • Thermal control by PerfuseCell Heating-Support-Foot and agitation by slim-line servo motor 
  • Thermal control by Eppendorf SciVario ThermoWell and the Maxon RE30 motor

General features of CellRetention™ 2 liter P-SUB with Magnetic-Bottom-Drive (MBD):

  • Headplate with 5 x PG13.5 circumference ports and 6 ports for In&Out with small diameter hoses, exhaust, temp well
  • With MBD the P-SUB mounts in PerfuseCell Heating-Support-Foot (for electrical thermal control) which is mounted onto the PerfuseCell Magnetic-Stirrer-Table (MST) for agitation by any servo motor

 

CellRetention 2 ATF HPD PG13 kit
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CellRetention-2 ATF HPD PG13 kit

Thalia TM60A SUP unified with the SepraPor 110 cm2 HFF and mounted in PG13 headplate port. P-SUB inserted in ID110 mm ThermoWell for thermal control with Maxon RE30 for Head-Plate-Drive controlled by SciVario. The DO WisiWell and Hamilton pH SUS pre-installed in the headplate. Direct-Fit connects HFF to Thalia for better flow performance and lower shear stress. See this link - stress free connections

Further 2 liter P-SUB features:

  • One pitch blade impeller
  • Membrane-Diffusion-Cage comprises 3 meter pico porous silicone for efficient aeration
  • 3 small diameter weldable hoses length 900 mm 
  • 1 gas inlet small short hose with sterile filter for aeration
  • permeate 900 mm weldable hose - for rinsing and permeate
  • 1 small short hose with swabable valve for bottom sampling
  • Optional exhaust gas cooler
  • Single-Use-Sensors (SUS) for DO, pH, temp, level - optional biomass with HSF
  • 1 exhaust hose with 26 cm2sterile filter - see Sterile-Filter-Heater
  • Thalia SUP exchanged broth volume fully programmable from Clotho Drive Unit mastering accurately velocity, volume, number of variable strokes – no guessing

The headplate PG 13.5 ports designed for:

  1. One port pre-installed with the OneFerm pH Single-Use-Sensor (SUS) K8 or VP
  2. One port pre-installed with the VisiWell Single-Use-Well (SUW) designed for VisiFerm (RUS) DO sensor
  3. extra PG 13.5 ports
  4. CellRetention PG13 ATF kit with either 110 cm2 or 170 cm2 HFF surface area and Thalia
  5. Center port options; with MBD free for a SUS, with HPD occupied by bearing assembly

Further customization – contact us

All products are patented by www.stobbe.group

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